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Title	T cell protocols [electronic resource] : development and activation / edited by Kelly P. Kearse
Publication	Totowa, N.J. : Humana Press, c2000

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Location	Call Number	Volume	Status
Internet			Online

Table of Contents
	Preface
	Contributors
Pt. I	Overview: for Chapters 2-15
	1	Insights into T-Cell Development Using Transgenic and Knockout Mice / M. Albert Basson, Rose Zamoyska	3
Pt. II	T Cell Development
	2	Isolation and Characterization of Murine Early Intrathymic Precursor Populations / Li Wu, Ken Shortman	25
	3	Differentiation of Mouse Thymocytes in Fetal Thymus Organ Culture / Yousuke Takahama	37
	4	Purification of Immature CD4[superscript +]CD8[superscript +] Thymocytes by Panning with Anti-CD8 Antibody / Terrence G. Gardner, Kelly P. Kearse	47
	5	Transfection and Transcription of Genes in Developing Thymocytes / Alison M. Michie, Juan Carlos Zuniga-Pflucker	55
	6	Analysis of CD4/CD8 Lineage Commitment by Pronase Treatment and Reexpression Assay / Harumi Suzuki	63
	7	Intrathymic Delivery of MHC Genes Using Recombinant Adenoviruses / Ronald Rooke, Christophe Benoist, Diane Mathis	69
	8	Quantitative Analysis of the Usage of Human T-Cell Receptor [alpha] and [beta] Chain Variable Regions by Reverse Dot-Blot Hybridization [et al.] / Takaji Matsutani, Takeshi Yoshioka, Yuji Tsuruta	81
	9	Flow Cytometric Detection of Cytoplasmic and Surface CD3-[epsilon] Expression in Developing T Cells / Karl J. Franek, Robert Chervenak	103
	10	Assessing Apoptosis of Developing T Cells by Flow Cytometry [et al.] / Caroline D. V. A. Bishop, Amy Jost, Elizabeth Crane	117
	11	Isolation of T-Cell Subsets by Magnetic Cell Sorting (MACS) / Luminita A. Stanciu, Ratko Djukanovic	133
	12	Isolation of T-Cell Antigens by Retrovirus-Mediated Expression Cloning / Toshio Kitamura, Yoshihiro Morikawa	143
	13	Flow Cytometric Analysis of Murine T Lymphocytes: A Practical Guide / Melanie S. Vacchio, Elizabeth W. Shores	153
	14	Production and Characterization of Immature Murine T-Lymphoma Cell Lines / Ellen R. Richie, Dawn A. Walker	177
	15	Production and Characterization of T Cell Hybridomas / Janice White, John Kappler, Philippa Marrack	185
Pt. III	Overview: for Chapters 17-29
	16	Something Happens: A Brief History of TCR Signal Transduction / John J. O'Shea	197
Pt. IV	T-Cell Activation
	17	Measurement of Phosphatidylinositol (PI) Hydrolysis in Activated T Lymphocytes / Seth Mark Berney, T. Prescott Atkinson	211
	18	Fluorescence Polarization as an Early Measure of T-Lymphocyte Stimulation [et al.] / Mordechai Deutsch, Naomi Zurgil, Menachem Kaufman	221
	19	Measurement of Lymphoproliferation at the Single-Cell Level by Flow Cytometry / Hans Gaines, Gunnel Biberfeld	243
	20	Digital Image Analysis of Lymphocyte Activation [et al.] / Bradley W. McIntyre, T. Kent Teague, Darren G. Woodside	257
	21	Induction of T-Cell Signaling by Immobilized Integrin Ligands / Jean Maguire van Seventer, Gijs A. van Seventer	265
	22	Cytotoxic T-Cell Adherence Assay (CAA) / Graham R. Leggatt, Jay A. Berzofsky	277
	23	Detecting Ubiquitinated T-Cell Antigen Receptor subunits by Immunoblotting / Allan M. Weissman	283
	24	Determination of CD45 Tyrosine Phosphatase Activity in T Lymphocytes / David H. W. Ng, Jackie Felberg, Pauline Johnson	289
	25	Measurement of Protein Tyrosine Phosphorylation in T-Cell Subsets by Flow Cytometry / Dariush Farahi Far, Bernard Rossi	301
	26	Biochemical Analysis of Activated T Lymphocytes: Protein Phosphorylation and Ras, ERK, and JNK Activation / Patrick E. Fields, Thomas F. Gajewski	307
	27	Activation of Heterotrimeric GTP-Binding Proteins Upon TCR/CD3 Engagement / Constantine D. Tsoukas, Jack Stanners, Keith A. Ching	319
	28	Generation of Human T Helper 1 and T Helper 2 Subsets from Peripheral Blood-Derived Naive CD4[superscript +] T Cells [et al.] / Ellen M. Palmer, Roshanak Tolouei Semnani, Bradford L. McRae	325
	29	Transcriptional Expression Analysis of T-Cell Activation by Multiplex Messenger Assay (MMA) [et al.] / Karine Bernard, Samuel Granjeaud, Genevieve Victorero	337
	Index		355

Notes	This ebook was purchased by BGSU through OhioLINK. An unlimited number of users can access this book at one time.
Bibliog.	Includes bibliographical references and index
Contents	pt. 1. Overview: for chapters 2-15 -- Insights into T-cell development using transgenic and knockout mice -- pt. 2. T cell development -- Isolation and characterizatin of murine early intrathymic precursor populations -- Differentiation of mouse thymocytes in fetal thymus organ culture -- Purification of immature CD4+CD8+ thymyocytes by panning with anti-CD8 antibody -- Transfection and transcription of genes in developing thymocytes -- analysis of CD4/CD8 lineage commitment by pronase treatment and reexpression assay -- Intrahymic delivery of MHC genes using recombinant adenoviruses -- Quantitative analysis of the usage of human T-cell receptor [alpha] and [beta] chain variable regions by reverse dot-blot hybridization -- Flow cytometric detection of cytoplasmic and surface CD3-� expression in developing T cells -- Assessing apoptosis in developing T cells by flow cytometry -- Isolation of T-cells subsets by magnetic cell sorting (MACS) -- Isolation of T-cell antigens by retrovirus-mediated expression cloning -- Flow cytometric analysis of murine T lymphocytes: a practical guide -- Production and characterization of T cell hybridomas -- pt. 3. Overview: for chapters 17-29 --Something happens: a brief history of TCR signal transduction -- pt. 4. T-cell activation -- Measurement of phosphatidylinositol (PI) hydrolysis in activated T lymphocytes -- Fluorescence polarization as an early measure of T-lymphocyte stimulation -- Measurement of lymphoproliferation at the single-cell level by flow cytometry -- Digital image analysis of lymphocyte activation -- Induction of T-cell signaling by immobilized integrin ligands -- Cytotoxic T-cell adherence assay (CAA) -- Detecting ubiquitinated T-cell antigen receptor subunits by immunoblotting -- Determination of CD45 tyrosine phosphatase activity in T lymphocytes -- Measurement of protein tyrosine phosphorylation in T-cell subsets by flow cytometry -- Biochemical analysis of activated T lymphocytes: protein phosphorylation and Ras, ERK and JNK activation -- Activation of heterotrimeric GTP-binding proteins upon TCR/CD3 engagement -- Generation of human T helper 1 and T helper 2 subsets form peripheral blood-derived naive CD4+ T cells -- Transcriptional expression analysis of T-cell activation by multiplex meassenger assay (MMA)
Access	Available to OhioLINK libraries
Reproduction note	Electronic resource. Berlin : Springer, 2008.
System Info	Mode of access: World Wide Web
OCLC #	261067146
Phys. Description	xiv, 365 p. : ill. ; 25 cm
Other author	Kearse, Kelly P
SERIES	Methods in molecular biology ; 134
	Methods in molecular biology (Clifton, N.J.) ; v. 134
ISBN	0896038106 (alk. paper)
	9780896038103 (alk. paper)
Subjects	T cells -- Laboratory manuals