| Wallace Henry Coulter - a Short Biography Wallace H. Coulter Foundation | |
| Foreword / Z. Darzynkiewicz | |
| Preface / U. Sack, A. Tarnok, G. Rothe | |
| Background and Methodological Principles | |
| Cytometry - a Definitive History of the Early Days / J. P. Robinson | 1 |
| Concept Developments in Flow Cytometry / G. Valet | 29 |
| Technical Background and Methodological Principles of Flow Cytometry / G. Rothe | 53 |
| Technical and Methodological Basics of Slide-Based Cytometry / A. Mittag, J. Bocsi, W. Laffers, A. O. H. Gerstner | 89 |
| Selection and Combination of Fluorescent Dyes / M. Schiemann, D. H. Busch | 107 |
| Fluorescence-Resonance Energy Transfer (FRET) / G. Vamosi, G. Vereb, A. Bodnar, K. Toth, N. Baudendistel, S. Sandor Damjanovich, J. Szollosi | 141 |
| Quality Control and Standardization / A. Mittag, D. Lenz, A. Tarnok | 159 |
| Flow-Cytometric Cell Sorting / M. Cross, V. Dobel | 178 |
| Characterization and Phenotyping of Cells | |
| Characterization of T-Lymphocytes / R. Mauerer, R. Gruber | 190 |
| T-Cell Receptor Repertoire Analysis by Flow Cytometry / D. Kabelitz, D. Wesch | 200 |
| Characterization of B-Lymphocytes / M. Schlesier, K. Warnatz | 211 |
| Characterization of Natural Killer Cells / R. Jacobs, R. E. Schmidt | 230 |
| Lymphocyte Subsets in the Peripheral Blood of Healthy Children / U. Sack, F. Gerling, A. Tarnok | 261 |
| Quantitative Assessment of Plasmacytoid and Myeloid Dendritic Cells / R. V. Sorg | 272 |
| Peripheral Blood Stem Cells / S. Fruehauf | 286 |
| Endothelial Progenitor Cells / J. C. Fischer | 305 |
| Mesenchymal Progenitor Cells / J. C. Fischer | 317 |
| Analysis of Cell Functions | |
| Determination of Cell Physiological Parameters: pH, Ca[superscript 2+], Glutathione, Transmembrane Potential / A. Tarnok, G. Rothe | 325 |
| Oxidative Burst, Phagocytosis and Expression of Adhesion Molecules / A. Lun, I. Schulze, J. Roesler, V. Wahn | 343 |
| Platelet Function and Thrombopoietic Activity / A. Ruf, S. Barlage | 377 |
| DNA and Proliferation Measurements in Flow Cytometry / G. Brockhoff | 390 |
| Multidrug Resistance / A. Dorn-Beineke, C. Keup | 426 |
| Flow-Cytometric Analysis of Intracellularly Stained Cytokines, Phosphoproteins, and Microbial Antigens / I. Lehmann, J. Lehmann | 459 |
| Detection of Antigen-Specific T-Cells using MHC Multimers or Functional Parameters / A. Scheffold, D. H. Busch, F. Kern | 476 |
| Diagnostic Indications | |
| Immunophenotyping CD4 T-Cells to Monitor HIV Disease / F. F. Mandy | 503 |
| Primary Immunodeficiency Diseases / I. Schulze, V. Wahn | 524 |
| Diagnostics for Common Variable Immunodeficiency Syndrome / K. Warnatz, M. Schlesier | 540 |
| Monitoring of Organ-Transplanted Patients / A. Lun, U. Sack | 558 |
| Flow-Cytometric Analysis of Cells from the Respiratory Tract: Bronchoalveolar Lavage Fluid and Induced Sputum / D. Loppow | 580 |
| Pathophysiology and Immune Monitoring of Sepsis / C. Meisel, C. Hoflich, H.-D. Volk | 600 |
| Flow-Cytometric Immunophenotyping of Acute Leukemias / R. Ratei, T. Nebe, R. Schabath, H.-D. Kleine, L. Karawajew, W.-D. Ludwig | 612 |
| Flow Cytometry in the Diagnosis of Non-Hodgkin's Lymphomas / M. Wattad | 642 |
| Paroxysmal Nocturnal Hemoglobinuria / M. Grunewald | 668 |
| Fetal Erythrocytes / P. Sedlmayr | 680 |
| Flow-Cytometric Cross-Match for the Determination of Platelet Antibodies / R. Lynen, M. Kohler, T. J. Legler | 687 |
| Residual Cells in Blood Products / M. Wotzel | 694 |
| Enrichment of Disseminated Tumor Cells / J. Bocsi, B. Molnar, A. Mittag, D. Lenz | 714 |
| Subject Index | 725 |