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Indoleamine 2,3-Dioxygenase 1
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Antibodies
CPTC-IDO1-1  [hide details]
Background| Characterization Data
CPTC-IDO1-2  [hide details]
Background| Characterization Data
Antigen
Indoleamine 2,3-Dioxygenase 1[hide details]
Background| Characterization Data| SOPs| Links
Antibody: CPTC-IDO1-1
Background
Catalog Number:
CPTC-IDO1-1
RRID:
AB_2617275
Target Antigen:
Indoleamine 2,3-Dioxygenase 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
11/14/2019
Antigen Recognition(s):
Recombinant Full-length
SOP:
  Antibody Production at Strategic Diagnostics Inc (92.8 KB)

Purchase
DSHB CPTC-IDO1-1
Characterization Data
  IHC NCI60
  IHC Tissue
  Indirect ELISA
  NCI 60 Protein Array
  Western Blot - Recombinant Protein or Peptide
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Antibody: CPTC-IDO1-2
Background
Catalog Number:
CPTC-IDO1-2
RRID:
AB_2617276
Target Antigen:
Indoleamine 2,3-Dioxygenase 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
07/28/2021
Antigen Recognition(s):
Recombinant Full-length
SOP:
  Antibody Production at Strategic Diagnostics Inc (92.8 KB)

Purchase
DSHB CPTC-IDO1-2
External Links
Human Protein Atlas CPTC-IDO1-2
Characterization Data
  IHC HPA
  IHC NCI60
  IHC Tissue
  Indirect ELISA
  NCI 60 Protein Array
  Western Blot - Recombinant Protein or Peptide
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Antigen: Indoleamine 2,3-Dioxygenase 1
Background
NCI Identification Number:
10898
Antigen Name:
Indoleamine 2,3-Dioxygenase 1
CPTC Name:
CPTC-IDO1
Keywords:
Indoleamine 2,3-Dioxygenase 1; Indoleamine-Pyrrole 2,3-Dioxygenase; IDO-1; INDO; IDO; Indoleamine-Pyrrole 2,3 Dioxygenase; Indolamine 2,3 Dioxygenase; Indole 2,3-Dioxygenase; EC 1.13.11.52; IDO1
Function:
This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.IDO1 (Indoleamine 2,3-Dioxygenase 1) is a Protein Coding gene. Diseases associated with IDO1 include Listeriosis and Trypanosomiasis. Among its related pathways are Histidine, lysine, phenylalanine, tyrosine, proline and tryptophan catabolism and superpathway of tryptophan utilization. Gene Ontology (GO) annotations related to this gene include heme binding and tryptophan 2,3-dioxygenase activity.Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:23103127, PubMed:25157255, PubMed:14502282, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885).
Chromosomal Localization:
8p11.21
Expression System:
E. Coli
NCBI Accession Number:
BC027882.1
UniProt Accession Number:
P14902
DNA Source:
OB : MHS1010-7430088
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
51130
Buffers:
PBS
Expressed Sequence:
SNAMAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLP
DLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHG
DVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYEN
MDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLL
KALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGL
VYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRR
YMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHL
QIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEK
SLLKEG
Native Sequence:
MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLI
ESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVR
KVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDV
LFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKAL
LEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE
GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMP
PAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIV
TKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLL
KEG
Calculated Isoelectric Point:
6.87
Molecular Weight:
45.33
Last Updated:
09/02/2020
Links
EnsEMBL - EnsEMBL IDO1
Entrez - Entrez IDO1
Uniprot - Uniprot P14902
Characterization Data
Gel

Click image to enlarge
CPTC-IDO1 PAGE
PAGE of IDO1 (rAg 10898) with molecular weight standards in lane 1
Characterization SOP Files
 Evaluation using SDS-PAGE (68.9 KB)
 Protein Concentration Measurement using BioRad Protein Assay (64.5 KB)
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SOPs
 BL21 Competant Cells (25.5 KB)
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