RASA1, CPTAC-1548 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RASA1	GAP, RASA

Sequence Length (AA)	Molecular Weight (Da)
1047	116403

Protein Name
Ras GTPase-activating protein 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MMAAEAGSEE	GGPVTAGAGG	GGAAAGSSAY	PAVCRVKIPA	ALPVAAAPYP
60	70	80	90	100
GLVETGVAGT	LGGGAALGSE	FLGAGSVAGA	LGGAGLTGGG	TAAGVAGAAA
110	120	130	140	150
GVAGAAVAGP	SGDMALTKLP	TSLLAETLGP	GGGFPPLPPP	PYLPPLGAGL
160	170	180	190	200
GTVDEGDSLD	GPEYEEEEVA	IPLTAPPTNQ	WYHGKLDRTI	AEERLRQAGK
210	220	230	240	250
SGSYLIRESD	RRPGSFVLSF	LSQMNVVNHF	RIIAMCGDYY	IGGRRFSSLS
260	270	280	290	300
DLIGYYSHVS	CLLKGEKLLY	PVAPPEPVED	RRRVRAILPY	TKVPDTDEIS
310	320	330	340	350
FLKGDMFIVH	NELEDGWMWV	TNLRTDEQGL	IVEDLVEEVG	REEDPHEGKI
360	370	380	390	400
WFHGKISKQE	AYNLLMTVGQ	VCSFLVRPSD	NTPGDYSLYF	RTNENIQRFK
410	420	430	440	450
ICPTPNNQFM	MGGRYYNSIG	DIIDHYRKEQ	IVEGYYLKEP	VPMQDQEQVL
460	470	480	490	500
NDTVDGKEIY	NTIRRKTKDA	FYKNIVKKGY	LLKKGKGKRW	KNLYFILEGS
510	520	530	540	550
DAQLIYFESE	KRATKPKGLI	DLSVCSVYVV	HDSLFGRPNC	FQIVVQHFSE
560	570	580	590	600
EHYIFYFAGE	TPEQAEDWMK	GLQAFCNLRK	SSPGTSNKRL	RQVSSLVLHI
610	620	630	640	650
EEAHKLPVKH	FTNPYCNIYL	NSVQVAKTHA	REGQNPVWSE	EFVFDDLPPD
660	670	680	690	700
INRFEITLSN	KTKKSKDPDI	LFMRCQLSRL	QKGHATDEWF	LLSSHIPLKG
710	720	730	740	750
IEPGSLRVRA	RYSMEKIMPE	EEYSEFKELI	LQKELHVVYA	LSHVCGQDRT
760	770	780	790	800
LLASILLRIF	LHEKLESLLL	CTLNDREISM	EDEATTLFRA	TTLASTLMEQ
810	820	830	840	850
YMKATATQFV	HHALKDSILK	IMESKQSCEL	SPSKLEKNED	VNTNLTHLLN
860	870	880	890	900
ILSELVEKIF	MASEILPPTL	RYIYGCLQKS	VQHKWPTNTT	MRTRVVSGFV
910	920	930	940	950
FLRLICPAIL	NPRMFNIISD	SPSPIAARTL	ILVAKSVQNL	ANLVEFGAKE
960	970	980	990	1000
PYMEGVNPFI	KSNKHRMIMF	LDELGNVPEL	PDTTEHSRTD	LSRDLAALHE
1010	1020	1030	1040	1047
ICVAHSDELR	TLSNERGAQQ	HVLKKLLAIT	ELLQQKQNQY	TKTNDVR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1548 Collapse assay details

Data source: Panorama

Official Gene Symbol: RASA1
Peptide Sequence: LLAITELLQQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 1026
Peptide End: 1036

CPTAC ID: CPTAC-1548
Peptide Molecular Mass: 1,268.7704
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

CPTAC-1538: PIK3R2. ALGATFGPLLLR	CPTAC-1539: PIK3R1. FSAASSDNTENLIK	CPTAC-1540: PDPK1. QLLLTEGPHLYYVDPVNK
CPTAC-1541: MAPK1. VADPDHDHTGFLTEYVATR	CPTAC-1542: MAPK3. IADPEHDHTGFLTEYVATR	CPTAC-1543: MAP2K2. ISELGAGNGGVVTK
CPTAC-1544: MAP2K1. ISELGAGNGGVVFK	CPTAC-1545: MAP2K1. LIHLEIKPAIR	CPTAC-1546: RAF1. TISNGFGFK
CPTAC-1547: ARAF. TQHC[+57.0]DPEHFPFPAPANAPLQR	CPTAC-1548: RASA1. LLAITELLQQK	CPTAC-1549: NRAS. SFADINLYR
CPTAC-1550: KRAS. SFEDIHHYR	CPTAC-1551: HRAS. LVVVGAGGVGK	CPTAC-1552: HRAS. SFEDIHQYR
CPTAC-1553: SOS2. LPGYSSAEYR	CPTAC-1554: SOS1. LPSADVYR	CPTAC-1555: SOS1. EYIQPVQLR
CPTAC-1556: PTPN11. SNPGDFTLSVR	CPTAC-1557: GAB1. LTGDPDVLEYYK	CPTAC-1558: GRB2. ATADDELSFK
CPTAC-1559: SHC1. EAISLVC[+57.0]EAVPGAK	CPTAC-1560: EGFR. LTQLGTFEDHFLSLQR	CPTAC-1561: ADAM17. NIYLNSGLTSTK
CPTAC-1562: SHOC2. LDSLTTLYLR	CPTAC-1563: SPRED1. VPHQEENGC[+57.0]ADFFIR	CPTAC-1564: DUSP6. DSTNLDVLEEFGIK
CPTAC-1565: DUSP4. GSVSLEQILPAEEEVR	CPTAC-1566: ERRFI1. NSPSLFPC[+57.0]APLC[+57.0]ER	CPTAC-1567: CBL. GTEPIVVDPFDPR
CPTAC-1568: PEBP1. VLTPTQVK	CPTAC-1569: PTPRE. TGTFIALSNILER	CPTAC-1570: SPRY4. LQHPLTILPIDQVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	5.8	3.8	3.3	19.1	6.7	10	20	7.7	10.5	15	15	15
y8 (1+)	4.4	4.3	3.5	24.2	5.3	9	24.6	6.8	9.7	15	15	15
y7 (1+)	4.6	3.8	3.4	20.5	5.7	9	21	6.9	9.6	15	15	15
sum	2.6	3.3	2.6	20.1	5.4	9	20.3	6.3	9.4	15	15	15

RASA1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1548 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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RASA1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1548 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments