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- Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications
- Harry Towbin, Theophil Staehelin, Julian Gordon
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, No. 9 (Sep., 1979), pp. 4350-4354
- Subjects: Developmental & Cell Biology, General Science
- Key terms: protein, nitrocellulose, ribosomal, polyacrylamide, antibody, blot, antigen, serum, electrophoretic, electrophoresis, ribosome, procedure, binding, conjugate, immobilize, peroxidase, sheet, saline, dimensional, electrophoretically, chicken
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Cited by 2806
CiteRank: 2
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2.
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- Hybridization of Denatured RNA and Small DNA Fragments Transferred to Nitrocellulose
- Patricia S. Thomas
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, No. 9 (Sep., 1980), pp. 5201-5205
- Subjects: General Science
- Key terms: nitrocellulose, glyoxal, hybridization, transfer, denature, paper, globin, fragment, lane, denaturation, agarose, hybridize, blot, nucleotide, buffer, alkali, hydroxide, retain, laevi, efficiently, fractionate, adult, using, probe
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Cited by 746
CiteRank: 8
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3.
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- Efficient Transfer of Large DNA Fragments from Agarose Gels to Diazobenzyloxymethyl-Paper and Rapid Hybridization by using Dextran Sulfate
- Geoffrey M. Wahl, Michael Stern, George R. Stark
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, No. 8 (Aug., 1979), pp. 3683-3687
- Subjects: Developmental & Cell Biology, General Science
- Key terms: dextran, sulfate, hybridization, fragment, probe, paper, agarose, cleave, strand, nitrocellulose, transfer, hybridize, label, sodium, digest, restriction, hamster, fractionate, buffer, electrophoresis, alkali, denhardt
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Cited by 402
CiteRank: 66
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4.
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- Internal Amino Acid Sequence Analysis of Proteins Separated by One-or Two-Dimensional Gel Electrophoresis after in situ Protease Digestion on Nitrocellulose
- Ruedi H. Aebersold, John Leavitt, Raul A. Saavedra, Leroy E. Hood, Stephen B. H. Kent
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 20 (Oct. 15, 1987), pp. 6970-6974
- Subjects: Developmental & Cell Biology, General Science
- Key terms: protein, nitrocellulose, peptide, sequence, ponceau, digestion, fragment, amino, protease, electrophoresis, electroblott, nadodso, cleavage, buffer, destain, myelin, amido, trypsin, analysis, separate, method, separation
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Cited by 66
CiteRank: 2953
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5.
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- Screening λgt Recombinant Clones by Hybridization to Single Plaques in situ
- W. David Benton, Ronald W. Davis
- Science, Vol. 196, No. 4286 (Apr. 8, 1977), pp. 180-182
- Subjects: Aquatic Sciences, Developmental & Cell Biology, General Science
- Key terms: phage, plaque, filter, recombinant, cloning, plate, nitrocellulose, immunity, clone, adsorption, hybridization, tetracycline, replica, percent, nucleic, plasmid, gene, method, tryptone, screening, linker
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Cited by 548
CiteRank: 636
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6.
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- Identification, Developmental Regulation, and Response to Heat Shock of Two Antigenically Related Forms of a Major Nuclear Envelope Protein in Drosophila Embryos: Application of an Improved Method for Affinity Purification of Antibodies Using Polypeptides Immobilized on Nitrocellulose Blots
- David E. Smith, Paul A. Fisher
- The Journal of Cell Biology, Vol. 99, No. 1 (Jul., 1984), pp. 20-28
- Subjects: Developmental & Cell Biology
- Key terms: nitrocellulose, drosophila, antibody, polypeptide, blot, nuclear, eluate, elution, monoclonal, phosphatase, probe, embryo, antiserum, antigen, purify, affinity, show, shock, staining, elute, protein
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Cited by 83
CiteRank: 2054
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7.
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- Induction of Interleukin 1 and Tumor Necrosis Factor by Mycobacterial Proteins: The Monocyte Western Blot
- R. S. Wallis, M. Amir-Tahmasseb, J. J. Ellner
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 9 (May, 1990), pp. 3348-3352
- Subjects: Developmental & Cell Biology, General Science, Health Sciences
- Key terms: tuberculosis, monocyte, filtrate, polymyxin, mycobacterial, nitrocellulose, cell, induction, mycobacteria, fraction, protein, cytokine, mononuclear, protease, medium, production, immunol, culture, antibody, electrophoresis, interleukin, western
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Cited by 19
CiteRank: 8602
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8.
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- Isolation and Partial Characterization of the Drosophila Alcohol Dehydrogenase Gene
- David A. Goldberg
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, No. 10 (Oct., 1980), pp. 5794-5798
- Subjects: General Science
- Key terms: drosophila, hybridize, nuclease, clone, sof, sequence, restriction, protein, mapping, bamhi, adult, nitrocellulose, hybridization, intervene, strain, locus, agarose, ecori, experiment, genetic, flies
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Cited by 73
CiteRank: 1417
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9.
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- Immunocytolocalization of Extensin in Developing Soybean Seed Coats by Immunogold-Silver Staining and by Tissue Printing on Nitrocellulose Paper
- Gladys I. Cassab, Joseph E. Varner
- The Journal of Cell Biology, Vol. 105, No. 6 (Dec., 1987), pp. 2581-2588
- Subjects: Botany & Plant Sciences, Developmental & Cell Biology
- Key terms: soybean, hydroxyproline, palisade, hourglass, cell, antibody, coat, wall, immunogold, stage, tissue, protein, hilum, nitrocellulose, carrot, plant, glycoprotein, seed, silver, staining, preimmune, serum, stain, extractable, alkaline
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Cited by 47
CiteRank: 9445
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10.
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- Purification and Mapping of Specific mRNAs by Hybridization-Selection and Cell-Free Translation
- Robert P. Ricciardi, Jacqueline S. Miller, Bryan E. Roberts
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, No. 10 (Oct., 1979), pp. 4927-4931
- Subjects: Developmental & Cell Biology, General Science
- Key terms: mrna, adenovirus, fragment, hybridization, nitrocellulose, polypeptide, hybrid, selection, cell, transcript, coordinate, agarose, sequence, coding, reticulocyte, translation, fractionate, select, cytoplasmic, strand, restriction, nadodso, encoding
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Cited by 82
CiteRank: 3164
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