DOI:​10.1016/j.jviromet.2016.11.014
Corpus ID: 20627218
A rapid assay for detection of Rose rosette virus using reverse transcription-recombinase polymerase amplification using multiple gene targets.
B. Babu, B. Washburn, +5 authors M. Paret
Published 2017
Biology, Medicine

Journal of virological methods
Rose rosette disease caused by Rose rosette virus (RRV; genus Emaravirus) is the most economically relevant disease of Knock Out® series roses in the U.S. As there are no effective chemical control… Expand
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Topics from this paper
Reverse Transcription
Ross river virus
Rosa
Disease Management
Transcript
Reverse Transcriptase Polymerase Chain Reaction
recombinase
Real-Time Polymerase Chain Reaction
Laboratory
Genus
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References
SHOWING 1-10 OF 39 REFERENCES
Rapid and specific detection of Yam mosaic virus by reverse-transcription recombinase polymerase amplification.
Gonçalo Silva, M. Bömer, C. Nkere, P. Kumar, S. Seal
Biology, MedicineJournal of virological methods
2015
TLDR
A novel assay for specific YMV detection is described that is based on isothermal reverse transcription-recombinase polymerase amplification (RT-exoRPA) that has been shown to be reproducible and able to detect as little as 14 pg/μl of purified RNA obtained from an YMMV-infected plant, a sensitivity equivalent to that obtained with the reverse transcript-polymerase chain reaction ( RT-PCR) in current general use. Expand
39 Citations
PDF
Rapid and sensitive detection of Little cherry virus 2 using isothermal reverse transcription-recombinase polymerase amplification.
T. Mekuria, Shulu Zhang, K. Eastwell
Biology, MedicineJournal of virological methods
2014
TLDR
An isothermal reverse transcription-recombinase polymerase amplification (RT-RPA) technique was developed using LChV2 coat protein specific primers and probe and is confirmed to be simple, fast, and specific. Expand
Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus.
M. Euler, Yongjie Wang, Oliver Nentwich, O. Piepenburg, F. Hufert, M. Weidmann
Biology, Medicine
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
2012
TLDR
The presented combination of one-step-RT-RPA and portable fluorescence reading device could be a useful tool for field or point of care diagnostics. Expand
114 Citations
Early Detection of Dengue Virus by Use of Reverse Transcription-Recombinase Polymerase Amplification
B. Teoh, S. Sam, +11 authors S. AbuBakar
Biology, MedicineJournal of Clinical Microbiology
2015
TLDR
The results from the study suggest that the RT-RPA assay is the most rapid molecular diagnostic tool available for the detection of DENV.Expand
70 Citations
PDF
Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP(®) using reverse transcription-recombinase polymerase amplification.
Shulu Zhang, M. Ravelonandro, +4 authors A. Vrient
Biology, MedicineJournal of virological methods
2014
TLDR
This is the first report describing the use of such an innovative technique to detect rapidly plant viruses affecting perennial crops using reverse transcription-recombinase polymerase amplification and the higher sensitivity of the AmplifyRP(®) to detect PPV when compared to the conventional ELISA and ImmunoStrip assays. Expand
80 Citations
Development of a Panel of Recombinase Polymerase Amplification Assays for Detection of Biothreat Agents
M. Euler, Yongjie Wang, +6 authors M. Weidmann
Medicine, BiologyJournal of Clinical Microbiology
2013
TLDR
The RPAs seem suitable for the implementation of syndromic panels onto microfluidic platforms, and showed comparable sensitivities to real-time RCR assays in these extracts. Expand
A new approach for diagnosis of bovine coronavirus using a reverse transcription recombinase polymerase amplification assay
H. Amer, A. Abd El Wahed, M. Shalaby, F. Almajhdi, F. Hufert, M. Weidmann
Biology, MedicineJournal of Virological Methods
2013
TLDR
The BCoV RT-RPA constitutes a suitable accurate, sensitive and rapid alternative to the common measures used for bovine coronavirus diagnosis, and the use of a portable fluorescence reading device extends its application potential to use in the field and point-of-care diagnosis. Expand
Rapid Detection of Shrimp White Spot Syndrome Virus by Real Time, Isothermal Recombinase Polymerase Amplification Assay
Xiaoming Xia, Yongxin Yu, M. Weidmann, Ying-jie Pan, Shuling Yan, Yongjie Wang
Biology, Medicine
PloS one

2014
TLDR
This study presents the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device and reveals more satisfactory performance than qPCR.Expand
53 Citations
PDF
Development of a Recombinase Polymerase Amplification Assay for the Detection of Pathogenic Leptospira
Ahmed A Ahmed, Hans van der Linden, R. Hartskeerl
Biology, Medicine
International journal of environmental research and public health
2014
TLDR
RPA presents a powerful tool for the early diagnosis of leptospirosis in humans and in animals and enables the detection of the causative agent in reservoirs and environment, and is a valuable adjunct to current tools for surveillance and early outbreak warning. Expand
41 Citations
PDF
Real-time PCR in virology.
I. Mackay, K. Arden, A. Nitsche
Biology, MedicineNucleic acids research
2002
TLDR
The background, advantages and limitations of real-time PCR are described, the literature as it applies to virus detection in the routine and research laboratory is reviewed and the technology discussed has been applied to other areas of microbiology as well as studies of gene expression and genetic disease. Expand
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