Biology, Materials Science Journal of materials chemistry. B
An in situ bioprinting method is developed that allows the printing of cells under true physiological conditions by applying self-assembling ultrashort peptides as bioinks and it is demonstrated that different nanomaterials can easily be synthesized or incorporated in the 3D biopprinted peptide scaffolds which opens up the possibility of functionalized 3D scaffolds.
This work exploits two bioorthogonal photochemistries to achieve reversible immobilization of bioactive full-length proteins with good spatial and temporal control within synthetic, cell-laden biomimetic scaffolds and accomplished reversible differentiation of human mesenchymal stem cells to osteoblasts in a spatially defined manner.
A hydrogel for 4D cell culture which allows user-defined stiffening of the cellular environment and presentation of bioadhesive cues in an orthogonal manner using light of different wavelengths is described.
A convenient method for tracking the hardness of cell scaffolds in a non-invasive manner via their color change is shown and this leads to a smart hydrogel scaffold with dynamically adjustable and in situ monitorable mechanical properties that could be used as a user-designable microarray for cell culture applications.
Materials Science, Engineering Journal of materials chemistry. B
Two-photon polymerization-based femtosecond laser 3D printing technology allows one to produce hydrogel structures with 100 nm resolution, and this article reviews the basics of this technique as well as some of its applications in tissue engineering.
This chapter discusses in each cases the chemical and mechanistic background, the kinetics of the reactions and the biological applicability together with the limiting factors, with a focus on those that have shown broad utility in biological systems.
This review encapsulates where recent advances appear to leave the ever-shifting state of the art in the cell microenvironment, and it highlights areas in which substantial potential and uncertainty remain.
An image-guided micropatterning method is demonstrated for generating biomimetic hydrogel scaffolds with two-photon laser scanning photolithography to pattern hydrogels that guide cellular organization by structurally and biochemically recapitulating complex vascular niche microenvironments with high pattern fidelity at the microscale.
Polymer-based hydrogels have emerged as a unique class of biomaterials that enable cells to be cultured in three dimensions within user-defined synthetic microenvironments by tuning the initial properties of these networks through the incorporation of physiologically relevant cues.
The use of light to trigger cell adhesion to synthetic materials by controlling the presentation of the bioadhesive arginine-glycine-aspartic acid (RGD) oligopeptide with photoresponsive molecules is used.
It is shown that the 3D invasion of primary human mesenchymal stem cells can be spatiotemporally controlled by micropatterning the hydrogel with desired extracellular matrix (ECM) proteins and growth factors.
A robust synthetic strategy is introduced where macromolecular precursors react via a copper-free click chemistry, allowing for the direct encapsulation of cells within click hydrogels for the first time and enables patterning of biological functionalities within the gel in real-time and with micron-scale resolution.
Through this approach, it is demonstrated that photodegradable tethers can be used to sequester peptides and proteins into hydrogel depots and release them in an externally controlled, predictable manner without compromising biological function.
This method for immobilizing biomolecules in 3D matrices can generally be applied to any optically clear hydrogel, offering a solution to construct scaffolds with programmed spatial features for tissue engineering applications.
This work has developed a method to spatially control the immobilization of different growth factors in distinct volumes in 3D hydrogels, and to specifically guide differentiation of stem/progenitor cells therein.