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Reverse Transcription

Known as: Reverse Transcription Process, Transcription, Reverse 
A DNA synthesis process that uses RNA as the initial template for synthesis of DNA, but which also includes an RNase activity to remove the RNA… 
National Institutes of Health

Related topics

Related topics

11 relations
DNARNARNA-Directed DNA PolymeraseReverse Transcriptase Polymerase Chain Reaction

Narrower (2)

RNA-directed DNA polymerase activityreverse transcription involved in RNA-mediated transposition

Papers overview

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Highly Cited
2010
Highly Cited
2010
Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR).
Review
2005
Review
2005
Real-time RT-PCR normalisation; strategies and considerations
Real-time RT-PCR has become a common technique, no longer limited to specialist core facilities. It is in many cases the only… 
Highly Cited
2004
Highly Cited
2004
Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data…
Accurate normalization is an absolute prerequisite for correct measurement of gene expression. For quantitative real-time reverse… 
Highly Cited
2003
Highly Cited
2003
A novel coronavirus associated with severe acute respiratory syndrome.
BACKGROUND A worldwide outbreak of severe acute respiratory syndrome (SARS) has been associated with exposures originating from a… 
Review
2002
Review
2002
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA… 
Review
2000
Review
2000
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.
The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low-abundance mRNA… 
Highly Cited
2000
Highly Cited
2000
Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods.
Four quantitative reverse transcription-PCR (RT-PCR) methods were compared to evaluate the time course of mRNA formation and… 
Highly Cited
1992
Highly Cited
1992
Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.
Effective methods are needed to identify and isolate those genes that are differentially expressed in various cells or under… 
Highly Cited
1988
Highly Cited
1988
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
We have devised a simple and efficient cDNA cloning strategy that overcomes many of the difficulties encountered in obtaining… 
Highly Cited
1982
Highly Cited
1982
Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediate
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